Recombination-mediated PCR-directed plasmid construction in vivo in yeast.
We have extended the technique of PCR-directed recombination in Saccharomyces cerevisiae to develop a simple method for plasmid or gene construction in the absence of suitable restriction sites. The DNA to be cloned is PCR-amplified with 30-40 bp of homology to a linearized yeast plasmid. Co-transfo...
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| Publicat a: | Nucleic Acids Res |
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| Autors principals: | , , , |
| Format: | Artigo |
| Idioma: | Inglês |
| Publicat: |
Oxford University Press
1997
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| Matèries: | |
| Accés en línia: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC146432/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/9016579/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/25.2.451 |
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