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In vitro selection of hammerhead ribozymes containing a bulged nucleotide in stem II.

Hammerhead ribozymes were transcribed from a dsDNA template containing four random nucleotides between stems II and III, which replace the naturally occurring GAA nucleotides. In vitro selection was used to select hammerhead ribozymes capable of in cis cleavage using denaturing polyacrylamide gels f...

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Udgivet i:Nucleic Acids Res
Principais autores: Thomson, J B, Sigurdsson, S T, Zeuch, A, Eckstein, F
Format: Artigo
Sprog:Inglês
Udgivet: Oxford University Press 1996
Fag:
Research Article
Online adgang:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC146259/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/8948630/
https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/24.22.4401
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