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A simple method for 3'-labeling of RNA.

We describe a simple method for 3'-end labeling RNAs of known sequence. A short DNA template is designed to anneal to the 3'-end of the RNA, with a two nucleotide 5' overhang of 3'-TA-5', 3'-TG-5' or 3'-TC-5'. The Klenow fragment of DNA polymerase I can t...

Disgrifiad llawn

Wedi'i Gadw mewn:
Manylion Llyfryddiaeth
Prif Awduron: Huang, Z, Szostak, J W
Fformat: Artigo
Iaith:Inglês
Cyhoeddwyd: 1996
Pynciau:
Mynediad Ar-lein:https://ncbi.nlm.nih.gov/pmc/articles/PMC146232/
https://ncbi.nlm.nih.gov/pubmed/8932396
Tagiau: Ychwanegu Tag
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