A fluorescence-labeling method for sequencing small RNA on polyacrylamide gel.

A practical fluorescence-labeling method for sequencing small RNAs by the traditional 'direct read out' on polyacrylamide gel electrophoresis was established. The 3' terminus of RNA was oxidized into dialdehyde by sodium periodate and then labeled with fluorescein-5-thiosemicarbazide through the con...

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Detalhes bibliográficos

Publicado no:	Nucleic Acids Res
Principais autores:	Wu, T P, Ruan, K C, Liu, W Y
Formato:	Artigo
Idioma:	Inglês
Publicado em:	Oxford University Press 1996
Assuntos:	Research Article
Acesso em linha:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC146087/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/8811106/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/24.17.3472
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