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Hyper-negative template DNA supercoiling during transcription of the tetracycline-resistance gene in topA mutants is largely constrained in vivo.

The excess linking deficit of plasmid DNA from topoisomerase I-defective bacteria (topA mutants) results mainly from transcription and is commonly ascribed to unbalanced relaxation of transcription-induced twin-supercoiled domains. This defect is aggravated in genes for membrane-binding proteins (su...

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Udgivet i:Nucleic Acids Res
Principais autores: Albert, A C, Spirito, F, Figueroa-Bossi, N, Bossi, L, Rahmouni, A R
Format: Artigo
Sprog:Inglês
Udgivet: Oxford University Press 1996
Fag:
Research Article
Online adgang:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC146055/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/8760899/
https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/24.15.3093
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