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Water dynamics and salt-activation of enzymes in organic media: Mechanistic implications revealed by NMR spectroscopy
Deuterium spin relaxation was used to examine the motion of enzyme-bound water on subtilisin Carlsberg colyophilized with inorganic salts for activation in different organic solvents. Spectral editing was used to ensure that the relaxation times were associated with relatively mobile deuterons, whic...
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| Autors principals: | , , , , , |
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| Format: | Artigo |
| Idioma: | Inglês |
| Publicat: |
National Academy of Sciences
2006
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| Matèries: | |
| Accés en línia: | https://ncbi.nlm.nih.gov/pmc/articles/PMC1458637/ https://ncbi.nlm.nih.gov/pubmed/16585507 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1073/pnas.0601113103 |
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