Sequence-specific labeling of superhelical DNA by triple helix formation and psoralen crosslinking.
Site-specific labeling of covalently closed circular DNA was achieved by using triple helix-forming oligonucleotides 10, 11 and 27 nt in length. The sequences consisted exclusively of pyrimidines (C and T) with a reactive psoralen at the 5'-end and a biotin at the 3'-end. The probes were directed to...
Guardat en:
| Publicat a: | Nucleic Acids Res |
|---|---|
| Autors principals: | , , , , |
| Format: | Artigo |
| Idioma: | Inglês |
| Publicat: |
Oxford University Press
1996
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| Matèries: | |
| Accés en línia: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC145834/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/8649989/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/24.9.1702 |
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