Sequence-specific labeling of superhelical DNA by triple helix formation and psoralen crosslinking.

Site-specific labeling of covalently closed circular DNA was achieved by using triple helix-forming oligonucleotides 10, 11 and 27 nt in length. The sequences consisted exclusively of pyrimidines (C and T) with a reactive psoralen at the 5'-end and a biotin at the 3'-end. The probes were directed to...

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Detalhes bibliográficos

Publicado no:	Nucleic Acids Res
Principais autores:	Pfannschmidt, C, Schaper, A, Heim, G, Jovin, T M, Langowski, J
Formato:	Artigo
Idioma:	Inglês
Publicado em:	Oxford University Press 1996
Assuntos:	Research Article
Acesso em linha:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC145834/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/8649989/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/24.9.1702
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