Modification of the Creator recombination system for proteomics applications – improved expression by addition of splice sites

BACKGROUND: Recombinational systems have been developed to rapidly shuttle Open Reading Frames (ORFs) into multiple expression vectors in order to analyze the large number of cDNAs available in the post-genomic era. In the Creator system, an ORF introduced into a donor vector can be transferred with...

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Détails bibliographiques
Auteurs principaux: Colwill, Karen, Wells, Clark D, Elder, Kelly, Goudreault, Marilyn, Hersi, Kadija, Kulkarni, Sarang, Hardy, W Rod, Pawson, Tony, Morin, Gregg B
Format: Artigo
Langue:Inglês
Publié: BioMed Central 2006
Sujets:
Methodology Article
Accès en ligne:https://ncbi.nlm.nih.gov/pmc/articles/PMC1421398/
https://ncbi.nlm.nih.gov/pubmed/16519801
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/1472-6750-6-13
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