Quantitative Detection of Neospora caninum in Bovine Aborted Fetuses and Experimentally Infected Mice by Real-Time PCR

We report the development of a real-time PCR assay for the quantitative detection of Neospora caninum in infected host tissues. The assay uses the double-stranded DNA-binding dye SYBR Green I to continuously monitor product formation. Oligonucleotide primers were designed to amplify a 76-bp DNA frag...

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Pubblicato in:	J Clin Microbiol
Autori principali:	Collantes-Fernández, Esther, Zaballos, Ángel, Álvarez-García, Gema, Ortega-Mora, Luis M.
Natura:	Artigo
Lingua:	Inglês
Pubblicazione:	American Society for Microbiology (ASM) 2002
Soggetti:	Clinical Veterinary Microbiology
Accesso online:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC140374/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/11923330/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/JCM.40.4.1194-1198.2002
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