Quantitative Detection of Neospora caninum in Bovine Aborted Fetuses and Experimentally Infected Mice by Real-Time PCR

We report the development of a real-time PCR assay for the quantitative detection of Neospora caninum in infected host tissues. The assay uses the double-stranded DNA-binding dye SYBR Green I to continuously monitor product formation. Oligonucleotide primers were designed to amplify a 76-bp DNA frag...

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Bibliographic Details
Main Authors: Collantes-Fernández, Esther, Zaballos, Ángel, Álvarez-García, Gema, Ortega-Mora, Luis M.
Format: Artigo
Language:Inglês
Published: American Society for Microbiology 2002
Subjects:
Clinical Veterinary Microbiology
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC140374/
https://ncbi.nlm.nih.gov/pubmed/11923330
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/JCM.40.4.1194-1198.2002
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