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Application of Real-Time Fluorescent PCR for Quantitative Assessment of Neospora caninum Infections in Organotypic Slice Cultures of Rat Central Nervous System Tissue

The previously described Nc5-specific PCR test for the diagnosis of Neospora caninum infections was used to develop a quantitative PCR assay which allows the determination of infection intensities within different experimental and diagnostic sample groups. The quantitative PCR was performed by using...

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Detalhes bibliográficos
Main Authors: Müller, Norbert, Vonlaufen, Nathalie, Gianinazzi, Christian, Leib, Stephen L., Hemphill, Andrew
Formato: Artigo
Idioma:Inglês
Publicado em: American Society for Microbiology 2002
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC120097/
https://ncbi.nlm.nih.gov/pubmed/11773124
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/JCM.40.1.252-255.2002
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