Application of Real-Time Fluorescent PCR for Quantitative Assessment of Neospora caninum Infections in Organotypic Slice Cultures of Rat Central Nervous System Tissue

The previously described Nc5-specific PCR test for the diagnosis of Neospora caninum infections was used to develop a quantitative PCR assay which allows the determination of infection intensities within different experimental and diagnostic sample groups. The quantitative PCR was performed by using...

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Pubblicato in:	J Clin Microbiol
Autori principali:	Müller, Norbert, Vonlaufen, Nathalie, Gianinazzi, Christian, Leib, Stephen L., Hemphill, Andrew
Natura:	Artigo
Lingua:	Inglês
Pubblicazione:	American Society for Microbiology (ASM) 2002
Soggetti:	Clinical Veterinary Microbiology
Accesso online:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC120097/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/11773124/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/JCM.40.1.252-255.2002
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