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Application of Real-Time Fluorescent PCR for Quantitative Assessment of Neospora caninum Infections in Organotypic Slice Cultures of Rat Central Nervous System Tissue

The previously described Nc5-specific PCR test for the diagnosis of Neospora caninum infections was used to develop a quantitative PCR assay which allows the determination of infection intensities within different experimental and diagnostic sample groups. The quantitative PCR was performed by using...

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Détails bibliographiques
Publié dans:J Clin Microbiol
Auteurs principaux: Müller, Norbert, Vonlaufen, Nathalie, Gianinazzi, Christian, Leib, Stephen L., Hemphill, Andrew
Format: Artigo
Langue:Inglês
Publié: American Society for Microbiology (ASM) 2002
Sujets:
Accès en ligne:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC120097/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/11773124/
https://ncbi.nlm.nih.govhttps://doi.org/10.1128/JCM.40.1.252-255.2002
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