Detection of Mammalian Reovirus RNA by Using Reverse Transcription-PCR: Sequence Diversity within the λ3-Encoding L1 Gene

Reoviruses infect virtually all mammalian species, and infection of humans is associated with mild gastrointestinal or upper respiratory illnesses. To improve reovirus detection strategies, we developed a reverse transcription-PCR technique to amplify a fragment of the reovirus L1 gene segment. This...

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Bibliographic Details
Main Authors: Leary, Thomas P., Erker, James C., Chalmers, Michelle L., Cruz, Andrea T., Wetzel, J. Denise, Desai, Suresh M., Mushahwar, Isa K., Dermody, Terence S.
Format: Artigo
Language:Inglês
Published: American Society for Microbiology 2002
Subjects:
Virology
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC140344/
https://ncbi.nlm.nih.gov/pubmed/11923358
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/JCM.40.4.1368-1375.2002
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