A Y639F/H784A T7 RNA polymerase double mutant displays superior properties for synthesizing RNAs with non-canonical NTPs

A T7 RNA polymerase in which Tyr639 is mutated to Phe readily utilizes 2′-deoxy, 2′-NH(2) and 2′-F NTPs as substrates and has been widely used to synthesize modified RNAs for a variety of applications. This mutant does not readily utilize NTPs with bulkier 2′-substituents, nor does it facilitate inc...

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Pubblicato in:	Nucleic Acids Res
Autori principali:	Padilla, Robert, Sousa, Rui
Natura:	Artigo
Lingua:	Inglês
Pubblicazione:	Oxford University Press 2002
Soggetti:	NAR Methods Online
Accesso online:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC140087/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/12490729/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/gnf138
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