Promoter-trapping in Saccharomyces cerevisiae by radiation-assisted fragment insertion

Non-homologous insertion (NHI) of DNA fragments into genomic DNA is a method widely used in insertional mutagenesis screens. In the yeast Saccharomyces cerevisiae, the efficiency of NHI is very low. Here we report that its efficiency can be increased by γ-irradiation of recipient cells at the time o...

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Bibliografiske detaljer

Udgivet i:	Nucleic Acids Res
Principais autores:	Kiechle, Markus, Manivasakam, Palaniyandi, Eckardt-Schupp, Friederike, Schiestl, Robert H., Friedl, Anna A.
Format:	Artigo
Sprog:	Inglês
Udgivet:	Oxford University Press 2002
Fag:	NAR Methods Online
Online adgang:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC140085/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/12490727/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/gnf136
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