Promoter-trapping in Saccharomyces cerevisiae by radiation-assisted fragment insertion
Non-homologous insertion (NHI) of DNA fragments into genomic DNA is a method widely used in insertional mutagenesis screens. In the yeast Saccharomyces cerevisiae, the efficiency of NHI is very low. Here we report that its efficiency can be increased by γ-irradiation of recipient cells at the time o...
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| Veröffentlicht in: | Nucleic Acids Res |
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| Hauptverfasser: | , , , , |
| Format: | Artigo |
| Sprache: | Inglês |
| Veröffentlicht: |
Oxford University Press
2002
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| Schlagworte: | |
| Online-Zugang: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC140085/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/12490727/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/gnf136 |
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