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Directed evolution of an extremely fast phosphotriesterase by in vitro compartmentalization
We describe the selection of a phosphotriesterase with a very fast k(cat) (over 10(5) s(–1)), 63 times higher than the already very efficient wild-type enzyme. The enzyme was selected from a library of 3.4 × 10(7) mutated phosphotriesterase genes using a novel strategy based on linking genotype and...
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| Main Authors: | , |
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| Format: | Artigo |
| Sprog: | Inglês |
| Udgivet: |
Oxford University Press
2003
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| Fag: | |
| Online adgang: | https://ncbi.nlm.nih.gov/pmc/articles/PMC140064/ https://ncbi.nlm.nih.gov/pubmed/12505981 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/emboj/cdg014 |
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