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Cloning, expression, purification, and characterization of LC-1 ScFv with GFP tag
Total RNA was isolated from the hybridoma cell line (LC-1), which secretes anti-lung adenocarcinoma monoclonal antibody, and was transferred into cDNA. Based on the FR1 (framework region 1) and FR4 conserved regions of LC-1 gene, the variable regions of heavy chain (Vh) and light chain (Vl) were amp...
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| Hoofdauteurs: | , , , |
|---|---|
| Formaat: | Artigo |
| Taal: | Inglês |
| Gepubliceerd in: |
Zhejiang University Press
2005
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| Onderwerpen: | |
| Online toegang: | https://ncbi.nlm.nih.gov/pmc/articles/PMC1389867/ https://ncbi.nlm.nih.gov/pubmed/16052719 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1631/jzus.2005.B0832 |
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