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Cloning, expression, purification, and characterization of LC-1 ScFv with GFP tag

Total RNA was isolated from the hybridoma cell line (LC-1), which secretes anti-lung adenocarcinoma monoclonal antibody, and was transferred into cDNA. Based on the FR1 (framework region 1) and FR4 conserved regions of LC-1 gene, the variable regions of heavy chain (Vh) and light chain (Vl) were amp...

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Detalhes bibliográficos
Main Authors: Lu, Min, Gong, Xing-guo, Yu, Hong, Li, Jian-yong
Formato: Artigo
Idioma:Inglês
Publicado em: Zhejiang University Press 2005
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC1389867/
https://ncbi.nlm.nih.gov/pubmed/16052719
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1631/jzus.2005.B0832
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