Monitoring mis-acylated tRNA suppression efficiency in mammalian cells via EGFP fluorescence recovery

A reporter assay was developed to detect and quantify nonsense codon suppression by chemically aminoacylated tRNAs in mammalian cells. It is based on the cellular expression of the enhanced green fluorescent protein (EGFP) as a reporter for the site-specific amino acid incorporation in its sequence...

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Bibliografische Detailangaben

Veröffentlicht in:	Nucleic Acids Res
Hauptverfasser:	Ilegems, Erwin, Pick, Horst M., Vogel, Horst
Format:	Artigo
Sprache:	Inglês
Veröffentlicht:	Oxford University Press 2002
Schlagworte:	NAR Methods Online
Online-Zugang:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC137983/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/12466560/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/gnf128
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