Monitoring mis-acylated tRNA suppression efficiency in mammalian cells via EGFP fluorescence recovery

A reporter assay was developed to detect and quantify nonsense codon suppression by chemically aminoacylated tRNAs in mammalian cells. It is based on the cellular expression of the enhanced green fluorescent protein (EGFP) as a reporter for the site-specific amino acid incorporation in its sequence...

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Detalhes bibliográficos

Publicado no:	Nucleic Acids Res
Principais autores:	Ilegems, Erwin, Pick, Horst M., Vogel, Horst
Formato:	Artigo
Idioma:	Inglês
Publicado em:	Oxford University Press 2002
Assuntos:	NAR Methods Online
Acesso em linha:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC137983/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/12466560/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/gnf128
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