Monitoring mis-acylated tRNA suppression efficiency in mammalian cells via EGFP fluorescence recovery

A reporter assay was developed to detect and quantify nonsense codon suppression by chemically aminoacylated tRNAs in mammalian cells. It is based on the cellular expression of the enhanced green fluorescent protein (EGFP) as a reporter for the site-specific amino acid incorporation in its sequence...

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Dades bibliogràfiques

Publicat a:	Nucleic Acids Res
Autors principals:	Ilegems, Erwin, Pick, Horst M., Vogel, Horst
Format:	Artigo
Idioma:	Inglês
Publicat:	Oxford University Press 2002
Matèries:	NAR Methods Online
Accés en línia:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC137983/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/12466560/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/gnf128
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