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Estimating the number of integrations in transformed plants by quantitative real-time PCR

BACKGROUND: When generating transformed plants, a first step in their characterization is to obtain, for each new line, an estimate of how many copies of the transgene have been integrated in the plant genome because this can deeply influence the level of transgene expression and the ease of stabili...

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Autors principals: Mason, Giovanna, Provero, Paolo, Vaira, Anna Maria, Accotto, Gian Paolo
Format: Artigo
Idioma:Inglês
Publicat: BioMed Central 2002
Matèries:
Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC137580/
https://ncbi.nlm.nih.gov/pubmed/12398792
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/1472-6750-2-20
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