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Standard Addition Quantitative Real-Time PCR (SAQPCR): A Novel Approach for Determination of Transgene Copy Number Avoiding PCR Efficiency Estimation

Quantitative real-time polymerase chain reaction (qPCR) has been previously applied to estimate transgene copy number in transgenic plants. However, the results can be erroneous owing to inaccurate estimation of PCR efficiency. Here, a novel qPCR approach, named standard addition qPCR (SAQPCR), was...

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Bibliografische gegevens
Hoofdauteurs: Huang, Yuji, Yin, Xueren, Zhu, Changqing, Wang, Weiwei, Grierson, Donald, Xu, Changjie, Chen, Kunsong
Formaat: Artigo
Taal:Inglês
Gepubliceerd in: Public Library of Science 2013
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Online toegang:https://ncbi.nlm.nih.gov/pmc/articles/PMC3538589/
https://ncbi.nlm.nih.gov/pubmed/23308234
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0053489
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