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Two-fold differences are the detection limit for determining transgene copy numbers in plants by real-time PCR
BACKGROUND: After transformation, plants that are homozygous and contain one copy of the transgene are typically selected for further study. If real-time PCR is to be used to determine copy number and zygosity, it must be able to distinguish hemizygous from homozygous and one-copy from two-copy plan...
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| Autores principales: | , , |
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| Formato: | Artigo |
| Lenguaje: | Inglês |
| Publicado: |
BioMed Central
2004
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| Materias: | |
| Acceso en línea: | https://ncbi.nlm.nih.gov/pmc/articles/PMC493272/ https://ncbi.nlm.nih.gov/pubmed/15251044 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/1472-6750-4-14 |
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