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Production of maternal-zygotic mutant zebrafish by germ-line replacement
We report a generally applicable strategy for transferring zygotic lethal mutations through the zebrafish germ line. By using a morpholino oligonucleotide that blocks primordial germ cell (PGC) development, we generate embryos devoid of endogenous PGCs to serve as hosts for the transplantation of ge...
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| Autors principals: | , , , , , , |
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| Format: | Artigo |
| Idioma: | Inglês |
| Publicat: |
National Academy of Sciences
2002
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| Matèries: | |
| Accés en línia: | https://ncbi.nlm.nih.gov/pmc/articles/PMC137520/ https://ncbi.nlm.nih.gov/pubmed/12397179 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1073/pnas.222459999 |
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