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Production of maternal-zygotic mutant zebrafish by germ-line replacement

We report a generally applicable strategy for transferring zygotic lethal mutations through the zebrafish germ line. By using a morpholino oligonucleotide that blocks primordial germ cell (PGC) development, we generate embryos devoid of endogenous PGCs to serve as hosts for the transplantation of ge...

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Библиографические подробности
Главные авторы: Ciruna, Brian, Weidinger, Gilbert, Knaut, Holger, Thisse, Bernard, Thisse, Christine, Raz, Erez, Schier, Alexander F.
Формат: Artigo
Язык:Inglês
Опубликовано: National Academy of Sciences 2002
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Online-ссылка:https://ncbi.nlm.nih.gov/pmc/articles/PMC137520/
https://ncbi.nlm.nih.gov/pubmed/12397179
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1073/pnas.222459999
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