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Novel thermostable Y-family polymerases: applications for the PCR amplification of damaged or ancient DNAs

For many years, Taq polymerase has served as the stalwart enzyme in the PCR amplification of DNA. However, a major limitation of Taq is its inability to amplify damaged DNA, thereby restricting its usefulness in forensic applications. In contrast, Y-family DNA polymerases, such as Dpo4 from Sulfolob...

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Bibliografiske detaljer
Main Authors: McDonald, John P., Hall, Ashley, Gasparutto, Didier, Cadet, Jean, Ballantyne, Jack, Woodgate, Roger
Format: Artigo
Sprog:Inglês
Udgivet: Oxford University Press 2006
Fag:
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC1373694/
https://ncbi.nlm.nih.gov/pubmed/16488882
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gkj512
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