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Analysis of site-specific protein–RNA cross-links in isolated RNP complexes, combining affinity selection and mass spectrometry

An important aspect of the assembly of RNPs, and in particular of spliceosomes, is the succession of proteins bound to any given site on the RNA. Protein–RNA cross-linking is a well-established technique for investigating this, but the identification of a cross-linked protein has so far relied upon...

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Autors principals: RHODE, BRITTA M., HARTMUTH, KLAUS, URLAUB, HENNING, LÜHRMANN, REINHARD
Format: Artigo
Idioma:Inglês
Publicat: Copyright 2003 by RNA Society 2003
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Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC1370507/
https://ncbi.nlm.nih.gov/pubmed/14624009
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1261/rna.5175703
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