A high-capacity RNA affinity column for the purification of human IRP1 and IRP2 overexpressed in Pichia pastoris

Regulated expression of proteins involved in mammalian iron metabolism is achieved in part through the interaction of the iron regulatory proteins IRP1 and IRP2 with highly conserved RNA stem-loop structures, known as iron-responsive elements (IREs), that are located within the 5′ or 3′ untranslated...

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Bibliografiske detaljer
Main Authors: ALLERSON, CHARLES R., MARTINEZ, ALAN, YIKILMAZ, EMINE, ROUAULT, TRACEY A.
Format: Artigo
Sprog:Inglês
Udgivet: Copyright 2003 by RNA Society 2003
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Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC1370403/
https://ncbi.nlm.nih.gov/pubmed/12592010
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1261/rna.2143303
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