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Purification and Characterization of Mammalian Glucose Transporters Expressed in Pichia Pastoris
The major bottleneck to the application of high-resolution techniques such as crystallographic X-ray diffraction and spectroscopic analyses to resolve the structure of mammalian membrane proteins has been the ectopic expression and purification of sufficient quantities of non-denatured proteins. Thi...
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| Hauptverfasser: | , |
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| Format: | Artigo |
| Sprache: | Inglês |
| Veröffentlicht: |
2009
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| Schlagworte: | |
| Online Zugang: | https://ncbi.nlm.nih.gov/pmc/articles/PMC2823836/ https://ncbi.nlm.nih.gov/pubmed/19883765 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.pep.2009.10.011 |
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