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Purification and Characterization of Mammalian Glucose Transporters Expressed in Pichia Pastoris

The major bottleneck to the application of high-resolution techniques such as crystallographic X-ray diffraction and spectroscopic analyses to resolve the structure of mammalian membrane proteins has been the ectopic expression and purification of sufficient quantities of non-denatured proteins. Thi...

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Detalhes bibliográficos
Main Authors: Alisio, Arturo, Mueckler, Mike
Formato: Artigo
Idioma:Inglês
Publicado em: 2009
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC2823836/
https://ncbi.nlm.nih.gov/pubmed/19883765
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.pep.2009.10.011
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