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Structural biology workflow for the expression and characterization of functional human sodium glucose transporter type 1 in Pichia pastoris
Heterologous expression of human membrane proteins is a challenge in structural biology towards drug discovery. Here we report a complete expression and purification process of a functional human sodium/D-glucose co-transporter 1 (hSGLT1) in Pichia pastoris as representative example of a useful stra...
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| Publicado no: | Sci Rep |
|---|---|
| Main Authors: | , , , , , , , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
Nature Publishing Group UK
2019
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC6362292/ https://ncbi.nlm.nih.gov/pubmed/30718602 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/s41598-018-37445-2 |
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