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In vivo selection of better self-splicing introns in Escherichia coli: the role of the P1 extension helix of the Tetrahymena intron.

In vivo selection was used to improve the activity of the Tetrahymena pre-rRNA self-splicing intron in the context of heterologous exons. The intron was engineered into a kanamycin nucleotidyltransferase gene, with the pairing between intron bases and the 5' and 3' splice sites maintained....

Deskribapen osoa

Gorde:
Xehetasun bibliografikoak
Egile Nagusiak: Guo, Feng, Cech, Thomas R
Formatua: Artigo
Hizkuntza:Inglês
Argitaratua: 2002
Gaiak:
Sarrera elektronikoa:https://ncbi.nlm.nih.gov/pmc/articles/PMC1370285/
https://ncbi.nlm.nih.gov/pubmed/12022231
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