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In vivo selection of better self-splicing introns in Escherichia coli: the role of the P1 extension helix of the Tetrahymena intron.

In vivo selection was used to improve the activity of the Tetrahymena pre-rRNA self-splicing intron in the context of heterologous exons. The intron was engineered into a kanamycin nucleotidyltransferase gene, with the pairing between intron bases and the 5' and 3' splice sites maintained....

詳細記述

保存先:
書誌詳細
主要な著者: Guo, Feng, Cech, Thomas R
フォーマット: Artigo
言語:Inglês
出版事項: 2002
主題:
オンライン・アクセス:https://ncbi.nlm.nih.gov/pmc/articles/PMC1370285/
https://ncbi.nlm.nih.gov/pubmed/12022231
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