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Evaluation of methylphosphonates as analogs for detecting phosphate contacts in RNA-protein complexes.

The well-studied interaction between the MS2 coat protein and its cognate hairpin was used to test the utility of the methylphosphonate linkage as a phosphate analog. A nitrocellulose filter binding assay was used to measure the change in binding affinity upon introduction of a single methylphosphon...

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Bibliographic Details
Main Authors: Dertinger, D, Uhlenbeck, O C
Format: Artigo
Language:Inglês
Published: 2001
Subjects:
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC1370115/
https://ncbi.nlm.nih.gov/pubmed/11345440
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