Mutagenesis of a stacking contact in the MS2 coat protein-RNA complex.

The thermodynamic contribution of a stacking interaction between Tyr85 in MS2 coat protein and a single-stranded pyrimidine in its RNA binding site has been examined. Mutation of Tyr85 to Phe, His, Cys, Ser and Ala decreased the RNA affinity by 1-3 kcal/mol under standard binding conditions. Since t...

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Bibliographic Details
Published in:EMBO J
Main Authors: LeCuyer, K A, Behlen, L S, Uhlenbeck, O C
Format: Artigo
Language:Inglês
Published: Nature Publishing Group 1996
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Research Article
Online Access:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC452510/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/9003760/
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