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The 100-kda U5 snRNP protein (hPrp28p) contacts the 5' splice site through its ATPase site.
To identify splicing factors in proximity of the 5' splice site (5'SS), we followed a crosslinking profile of site-specifically modified, photoreactive RNA substrates. Upon U4/U5/U6 snRNP addition, the 5'SS RNA crosslinks in an ATP-dependent manner to U6 snRNA, an unidentified protein...
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Auteurs principaux: | , , , |
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Format: | Artigo |
Langue: | Inglês |
Publié: |
2001
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Sujets: | |
Accès en ligne: | https://ncbi.nlm.nih.gov/pmc/articles/PMC1370077/ https://ncbi.nlm.nih.gov/pubmed/11233976 |
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