Identification of preferred protein interactions by phage-display of the human Src homology-3 proteome

We have determined the human genome to contain 296 different Src homology-3 (SH3) domains and cloned them into a phage-display vector. This provided a powerful and unbiased system for simultaneous assaying of the complete human SH3 proteome for the strongest binding to target proteins of interest, w...

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Detalhes bibliográficos
Main Authors: Kärkkäinen, Satu, Hiipakka, Marita, Wang, Jing-Huan, Kleino, Iivari, Vähä-Jaakkola, Marika, Renkema, G Herma, Liss, Michael, Wagner, Ralf, Saksela, Kalle
Formato: Artigo
Idioma:Inglês
Publicado em: 2006
Assuntos:
Scientific Report
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC1369250/
https://ncbi.nlm.nih.gov/pubmed/16374509
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/sj.embor.7400596
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