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Temperature Dependence and Thermodynamics of Klenow Polymerase Binding to Primed-Template DNA
DNA binding of Klenow polymerase has been characterized with respect to temperature to delineate the thermodynamic driving forces involved in the interaction of this polymerase with primed-template DNA. The temperature dependence of the binding affinity exhibits distinct curvature, with tightest bin...
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| Main Authors: | , , , |
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| Format: | Artigo |
| Language: | Inglês |
| Published: |
Biophysical Society
2006
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| Subjects: | |
| Online Access: | https://ncbi.nlm.nih.gov/pmc/articles/PMC1367323/ https://ncbi.nlm.nih.gov/pubmed/16339886 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1529/biophysj.105.071837 |
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