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Temperature Dependence and Thermodynamics of Klenow Polymerase Binding to Primed-Template DNA

DNA binding of Klenow polymerase has been characterized with respect to temperature to delineate the thermodynamic driving forces involved in the interaction of this polymerase with primed-template DNA. The temperature dependence of the binding affinity exhibits distinct curvature, with tightest bin...

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Detalles Bibliográficos
Autores principales: Datta, Kausiki, Wowor, Andy J., Richard, Allison J., LiCata, Vince J.
Formato: Artigo
Lenguaje:Inglês
Publicado: Biophysical Society 2006
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Acceso en línea:https://ncbi.nlm.nih.gov/pmc/articles/PMC1367323/
https://ncbi.nlm.nih.gov/pubmed/16339886
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1529/biophysj.105.071837
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