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Stepwise Unfolding of Ankyrin Repeats in a Single Protein Revealed by Atomic Force Microscopy

Using single-molecule atomic force microscopy, we find that a protein consisting of six identical ankyrin repeat units flanked by N- and C-terminal modules (N6C) unfolds in a stepwise, unit-by-unit fashion under a mechanical force. Stretching a N6C molecule results in a sawtooth pattern fingerprint,...

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Detalhes bibliográficos
Main Authors: Li, Lewyn, Wetzel, Svava, Plückthun, Andreas, Fernandez, Julio M.
Formato: Artigo
Idioma:Inglês
Publicado em: Biophysical Society 2006
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC1367297/
https://ncbi.nlm.nih.gov/pubmed/16387766
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1529/biophysj.105.078436
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