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Differential Interaction of Cardiac, Skeletal Muscle, and Yeast Tropomyosins with Fluorescent (Pyrene(235)) Yeast Actin
To monitor binding of tropomyosin to yeast actin, we mutated S(235) to C and labeled the actin with pyrene maleimide at both C(235) and the normally reactive C(374). Saturating cardiac tropomyosin (cTM) caused about a 20% increase in pyrene fluorescence of the doubly labeled F-actin but no change in...
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| Main Authors: | , , , |
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| Format: | Artigo |
| Jezik: | Inglês |
| Izdano: |
Biophysical Society
2006
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| Teme: | |
| Online dostop: | https://ncbi.nlm.nih.gov/pmc/articles/PMC1367282/ https://ncbi.nlm.nih.gov/pubmed/16326906 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1529/biophysj.105.064634 |
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