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Influence of Substrate Binding on the Mechanical Stability of Mouse Dihydrofolate Reductase
We investigated the effect of substrate binding on the mechanical stability of mouse dihydrofolate reductase using single-molecule force spectroscopy by atomic force microscopy. We find that under mechanical forces dihydrofolate reductase unfolds via a metastable intermediate with lifetimes on the m...
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| Main Authors: | , , , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
Biophysical Society
2005
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC1366864/ https://ncbi.nlm.nih.gov/pubmed/16183885 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1529/biophysj.105.072066 |
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