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Accessibility and Dynamics of Nitroxide Side Chains in T4 Lysozyme Measured by Saturation Recovery EPR

Long pulse saturation recovery electron paramagnetic resonance spectroscopy is applied to the investigation of spin-labeled side chains placed along a regular helix extending from 128 to 135 in T4 lysozyme. Under an argon atmosphere, analysis of the exponential saturation recovery curves gives the s...

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Detalhes bibliográficos
Main Authors: Pyka, Janusz, Ilnicki, Jan, Altenbach, Christian, Hubbell, Wayne L., Froncisz, Wojciech
Formato: Artigo
Idioma:Inglês
Publicado em: Biophysical Society 2005
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC1366708/
https://ncbi.nlm.nih.gov/pubmed/15994892
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1529/biophysj.105.059055
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