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Assessing Oligomerization of Membrane Proteins by Four-Pulse DEER: pH-Dependent Dimerization of NhaA Na(+)/H(+) Antiporter of E. coli
The pH dependence of the structure of the main Na(+)/H(+) antiporter NhaA of Escherichia coli is studied by continuous-wave (CW) and pulse electron paramagnetic resonance (EPR) techniques on singly spin-labeled mutants. Residues 225 and 254 were selected for site-directed spin labeling, as previous...
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| Main Authors: | , , , , , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
Biophysical Society
2005
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC1366617/ https://ncbi.nlm.nih.gov/pubmed/15894644 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1529/biophysj.105.062232 |
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