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Engineering a rare-cutting restriction enzyme: genetic screening and selection of NotI variants
Restriction endonucleases (REases) with 8-base specificity are rare specimens in nature. NotI from Nocardia otitidis-caviarum (recognition sequence 5′-GCGGCCGC-3′) has been cloned, thus allowing for mutagenesis and screening for enzymes with altered 8-base recognition and cleavage activity. Variants...
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| 主要な著者: | , , , , , |
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| フォーマット: | Artigo |
| 言語: | Inglês |
| 出版事項: |
Oxford University Press
2006
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| 主題: | |
| オンライン･アクセス: | https://ncbi.nlm.nih.gov/pmc/articles/PMC1360745/ https://ncbi.nlm.nih.gov/pubmed/16456032 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gkj483 |
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