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Tube-Gel Digestion: A NOVEL PROTEOMIC APPROACH FOR HIGH THROUGHPUT ANALYSIS OF MEMBRANE PROTEINS
This study describes a new protein digestion protocol in which a variety of detergents can be used to solubilize membrane proteins and facilitate trypsin digestion with higher efficiency. In this protocol, proteins are dissolved in solutions containing various detergents and directly incorporated in...
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Main Authors: | , |
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Formato: | Artigo |
Idioma: | Inglês |
Publicado em: |
2005
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Assuntos: | |
Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC1360194/ https://ncbi.nlm.nih.gov/pubmed/16150870 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1074/mcp.M500138-MCP200 |
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