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High-throughput expression and purification of membrane proteins

High-throughput (HT) methodologies have had a tremendous impact on structural biology of soluble proteins. High-resolution structure determination relies on the ability of the macromolecule to form ordered crystals that diffract X-rays. While crystallization remains somewhat empirical, for a given p...

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Detalhes bibliográficos
Main Authors: Mancia, Filippo, Love, James
Formato: Artigo
Idioma:Inglês
Publicado em: 2010
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC2933282/
https://ncbi.nlm.nih.gov/pubmed/20394823
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.jsb.2010.03.021
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